Hotspot

rps16-trnQ-UUG

LSC · 6631-7365 · 735 bp

Back to Alocasia

Pi 0.0924

MarkerSeek score 84.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 63
pi: 0.0924
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	808-1476 bp	1.0	86.4
rps16-trnQ-UUG_p2	TGATCACGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	822-1490 bp	1.0	84.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	812-1480 bp	1.0	81.6
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	800-1468 bp	1.0	81.3
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	807-1475 bp	1.0	80.6