Hotspot

trnH-GUG-psbA

LSC · 62-550 · 489 bp

Back to Alocasia

Pi 0.1

MarkerSeek score 81.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 48
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTGATCCA	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	552-690 bp	1.0	84.0
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	551-689 bp	1.0	84.0
trnH-GUG-psbA_p3	ACAATCCACTGCCTTGATCC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	553-691 bp	1.0	84.0
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GCTGCTGTTGAAGTTCCATC	50.0	50.0	57.9	57.4	551-689 bp	1.0	81.3
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	GCTGCTGTTGAAGTTCCATC	50.0	50.0	57.9	57.4	550-688 bp	1.0	81.3