Hotspot

ndhF

SSC · 115515-117770 · 2256 bp

Back to Alnus

Pi 0.00823
MarkerSeek score 46.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2256
variable_sites
106
pi
0.00823
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGAAGGGGACTAAACAGGA AGCTGGTGAATCAGAAACAGT 50.0 42.9 58.0 57.5 2652-2840 bp 0.973684 75.4
ndhF_p2 CAGAGTACCGTCACCCATTG AGCTGGTGAATCAGAAACAGT 55.0 42.9 58.0 57.5 2761-2949 bp 0.973684 75.3
ndhF_p3 AAGAAAGCGGGGAAAGTGAG AGCTGGTGAATCAGAAACAGT 50.0 42.9 58.1 57.5 2708-2896 bp 0.973684 74.6
ndhF_p4 GAAGAAAGCGGGGAAAGTGA AGCTGGTGAATCAGAAACAGT 50.0 42.9 58.1 57.5 2709-2897 bp 0.973684 74.6
ndhF_p5 GGAACAAGAGGAATCCACCG AGCTGGTGAATCAGAAACAGT 55.0 42.9 58.3 57.5 2635-2823 bp 0.973684 73.4