Hotspot

ndhA

SSC · 125804-128053 · 2250 bp

Back to Alnus

Pi 0.00706
MarkerSeek score 45.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2250
variable_sites
78
pi
0.00706
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGCAGCTCGTACTGTTTGTT AACAGGCGTTGGAAGGAATT 45.0 45.0 58.1 58.0 2740-2787 bp 1.0 83.0
ndhA_p2 TTGCAGCTCGTACTGTTTGT AACAGGCGTTGGAAGGAATT 45.0 45.0 58.1 58.0 2741-2788 bp 1.0 83.0
ndhA_p3 TGACCCCAGTTACCATAGGG AACAGGCGTTGGAAGGAATT 55.0 45.0 58.1 58.0 2708-2755 bp 1.0 82.6
ndhA_p4 TGCAGCTCGTACTGTTTGTT TGGGAAGTCCAATGGCAAAA 45.0 45.0 58.1 57.9 2828-2875 bp 1.0 82.2
ndhA_p5 TTGCAGCTCGTACTGTTTGT TGGGAAGTCCAATGGCAAAA 45.0 45.0 58.1 57.9 2829-2876 bp 1.0 82.2