Hotspot

trnF-GAA-ndhJ

LSC · 52661-53476 · 816 bp

Back to Alnus

Pi 0.023
MarkerSeek score 65.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
816
variable_sites
67
pi
0.023
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATGCCGGAAAGTTGGATAGG 55.0 50.0 58.0 57.7 891-998 bp 1.0 87.0
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATGCCGGAAAGTTGGATAGG 50.0 50.0 58.1 57.7 855-962 bp 1.0 86.9
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA ATGCCGGAAAGTTGGATAGG 55.0 50.0 57.9 57.7 879-986 bp 1.0 86.1
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA GCCGGAAAGTTGGATAGGAT 55.0 50.0 58.0 57.4 889-996 bp 1.0 84.7
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA GCCGGAAAGTTGGATAGGAT 50.0 50.0 58.1 57.4 853-960 bp 1.0 84.6