Hotspot

ycf3-trnS-GGA

LSC · 48825-49250 · 426 bp

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Pi 0.0201
MarkerSeek score 60.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
426
variable_sites
27
pi
0.0201
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CCATTTATCCGAGATCTGGGC TTCCAATGCTACGCCTTGAA 52.4 45.0 58.3 57.8 486-511 bp 1.0 74.0
ycf3-trnS-GGA_p2 CCATTTATCCGAGATCTGGGC ACGGAAAGAGAGGGATTCGA 52.4 50.0 58.3 58.2 536-561 bp 1.0 73.7
ycf3-trnS-GGA_p3 CATTTATCCGAGATCTGGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 57.6 57.8 485-510 bp 1.0 73.1
ycf3-trnS-GGA_p4 CATTTATCCGAGATCTGGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 57.6 58.2 535-560 bp 1.0 72.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 609-634 bp 1.0 71.2