Hotspot

atpH-atpI

LSC · 15747-17024 · 1278 bp

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Pi 0.00883
MarkerSeek score 63.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1278
variable_sites
59
pi
0.00883
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.0 57.9 1316-1399 bp 1.0 85.4
atpH-atpI_p2 GATCCCTTCTACAGCTTGGC TTTTGCAACTTTAGCTGCGG 55.0 45.0 58.0 57.9 1401-1484 bp 1.0 85.0
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.2 57.9 1317-1400 bp 1.0 84.2
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT AGGCGAATCTATGGAGGGTT 45.0 50.0 58.0 58.2 1337-1338 bp 0.052632 40.4
atpH-atpI_p5 GATCCCTTCTACAGCTTGGC AGGCGAATCTATGGAGGGTT 55.0 50.0 58.0 58.2 1422-1423 bp 0.052632 40.4