Hotspot

trnL-CAA-ycf15

IRa · 145499-145867 · 369 bp

Back to Alniphyllum

Pi 0.0136

MarkerSeek score 61.70

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 369
variable_sites: 5
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	TCTTTCGGATAGGCCCTGAA	50.0	50.0	58.0	58.1	500 bp	1.0	79.7
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	TCTTTCGGATAGGCCCTGAA	50.0	50.0	58.0	58.1	502 bp	1.0	79.7
trnL-CAA-ycf15_p3	AAATGGTAGACACGCGAGAC	TTCTCTTTCGGATAGGCCCT	50.0	50.0	58.0	57.8	503 bp	1.0	79.3
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	TTCTCTTTCGGATAGGCCCT	50.0	50.0	58.0	57.8	505 bp	1.0	79.3
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	TCTTTCGGATAGGCCCTGAA	50.0	50.0	58.1	58.1	512 bp	1.0	79.1