Hotspot

ycf15-trnL-CAA

IRb · 93228-93596 · 369 bp

Back to Alniphyllum

Pi 0.0136

MarkerSeek score 61.70

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 369
variable_sites: 5
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	TCTTTCGGATAGGCCCTGAA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.1	58.0	500 bp	1.0	79.7
ycf15-trnL-CAA_p2	TCTTTCGGATAGGCCCTGAA	TGAAATGGTAGACACGCGAG	50.0	50.0	58.1	58.0	502 bp	1.0	79.7
ycf15-trnL-CAA_p3	TTCTCTTTCGGATAGGCCCT	AAATGGTAGACACGCGAGAC	50.0	50.0	57.8	58.0	503 bp	1.0	79.3
ycf15-trnL-CAA_p4	TTCTCTTTCGGATAGGCCCT	TGAAATGGTAGACACGCGAG	50.0	50.0	57.8	58.0	505 bp	1.0	79.3
ycf15-trnL-CAA_p5	TCTTTCGGATAGGCCCTGAA	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.1	58.1	512 bp	1.0	79.1