Hotspot

psbT-psbN

LSC · 73699-73762 · 64 bp

Back to Alniphyllum

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	TGTATACCGCTTTTGGGCAA	47.6	45.0	57.6	57.8	168 bp	1.0	67.7
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	TTGTATACCGCTTTTGGGCA	47.6	45.0	57.6	57.8	169 bp	1.0	67.7
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	47.6	50.0	57.6	57.8	123 bp	1.0	67.6
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	47.6	50.0	57.6	57.8	124 bp	1.0	67.5
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	47.6	55.0	57.6	58.3	126 bp	1.0	66.8