Hotspot

petA-psbJ

LSC · 62647-63677 · 1031 bp

Back to Alniphyllum

Pi 0.00487

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 5
pi: 0.00487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1171-1174 bp	1.0	76.3
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1172-1175 bp	1.0	76.2
petA-psbJ_p3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1166-1169 bp	1.0	73.5
petA-psbJ_p4	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1165-1168 bp	1.0	73.1
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCCGGATTGGG	50.0	47.6	58.2	57.8	1179-1182 bp	1.0	69.4