Hotspot

accD-psaI

LSC · 57937-58718 · 782 bp

Back to Alniphyllum

Pi 0.00528

MarkerSeek score 63.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 4
pi: 0.00528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	887-906 bp	1.0	73.6
accD-psaI_p2	GAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	56.9	57.9	893-912 bp	1.0	73.0
accD-psaI_p3	GAGTTATTTCAGCTCCACGC	GCCGGAAATACTAGGCCTAC	50.0	55.0	56.9	57.5	876-895 bp	1.0	70.2
accD-psaI_p4	CGCTTTCGTTCCTTTGAATCA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.5	58.0	870-889 bp	1.0	70.1
accD-psaI_p5	CGCTTTCGTTCCTTTGAATCA	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.5	57.9	876-895 bp	1.0	69.5