Hotspot

rpl20-rps12

LSC · 72772-73543 · 772 bp

Back to Alniaria

Pi 0.00345

MarkerSeek score 61.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 4
pi: 0.00345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	926-928 bp	1.0	75.3
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	925-927 bp	1.0	75.3
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	889-891 bp	1.0	73.8
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	888-890 bp	1.0	73.8
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	50.0	50.0	57.9	58.8	887-889 bp	1.0	73.0