Hotspot

rps16-trnQ-UUG

LSC · 6392-7222 · 831 bp

Back to Alniaria

Pi 0.00577

MarkerSeek score 63.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 7
pi: 0.00577
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	889-915 bp	1.0	81.0
rps16-trnQ-UUG_p2	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	866-892 bp	1.0	79.3
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	899-925 bp	1.0	79.2
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	900-926 bp	1.0	79.2
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	951-977 bp	1.0	78.6