Hotspot

trnK-UUU-rps16

LSC · 4277-5257 · 981 bp

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Pi 0.00951
MarkerSeek score 68.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
981
variable_sites
14
pi
0.00951
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1045-1097 bp 1.0 82.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1044-1096 bp 1.0 82.0
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGGAACTTCGCCCTAATCAA 50.0 50.0 58.3 58.0 1054-1106 bp 1.0 81.1
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1100-1152 bp 1.0 80.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1099-1151 bp 1.0 80.5