Hotspot

ndhD

SSC · 109997-111499 · 1503 bp

Back to Alloteropsis

Pi 0.0142

MarkerSeek score 54.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 32
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAACACCTCTTTTCCCGCAT	TTTAGGGCCTGAAACAACCC	45.0	50.0	58.0	58.0	2236-2241 bp	1.0	82.3
ndhD_p2	AACACCTCTTTTCCCGCATT	TTTAGGGCCTGAAACAACCC	45.0	50.0	58.0	58.0	2235-2240 bp	1.0	82.3
ndhD_p3	ACACCTCTTTTCCCGCATTT	TTTAGGGCCTGAAACAACCC	45.0	50.0	58.0	58.0	2234-2239 bp	1.0	82.3
ndhD_p4	AACACCTCTTTTCCCGCATT	AAACCATTGCTTCGTCTGGT	45.0	45.0	58.0	58.0	2550-2552 bp	1.0	82.2
ndhD_p5	ACACCTCTTTTCCCGCATTT	AAACCATTGCTTCGTCTGGT	45.0	45.0	58.0	58.0	2549-2551 bp	1.0	82.2