Hotspot

rpl14-rpl16

LSC · 79147-79254 · 108 bp

Back to Alloteropsis

Pi 0.0556

MarkerSeek score 72.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 9
pi: 0.0556
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AAGGGTCTGAGGTTGAATCA	TAGCTGCCAGTAAAATGCCC	45.0	50.0	56.0	58.2	182-183 bp	1.0	66.4
rpl14-rpl16_p2	AAAGGGTCTGAGGTTGAATCA	TAGCTGCCAGTAAAATGCCC	42.9	50.0	56.8	58.2	183-184 bp	1.0	64.7
rpl14-rpl16_p3	AGGGTCTGAGGTTGAATCAT	TAGCTGCCAGTAAAATGCCC	45.0	50.0	55.8	58.2	181-182 bp	1.0	64.5
rpl14-rpl16_p4	AAGGGTCTGAGGTTGAATCAT	TAGCTGCCAGTAAAATGCCC	42.9	50.0	56.6	58.2	182-183 bp	1.0	62.9
rpl14-rpl16_p5	AAAAGGGTCTGAGGTTGAATCA	TAGCTGCCAGTAAAATGCCC	40.9	50.0	57.5	58.2	184-185 bp	1.0	62.4