Hotspot

trnF-GAA-ndhJ

LSC · 49594-50176 · 583 bp

Back to Alloteropsis

Pi 0.0369

MarkerSeek score 75.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 30
pi: 0.0369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	58.1	57.9	647-669 bp	1.0	62.2
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	ACGTAAGGACTATATAACCCCCA	50.0	43.5	58.1	57.9	700-722 bp	1.0	61.4
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.8	57.9	698-720 bp	1.0	60.5
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.5	57.9	650-672 bp	1.0	58.9
trnF-GAA-ndhJ_p5	AGGGTCAGGATAGCTCAGTT	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.4	57.9	689-711 bp	1.0	57.7