Hotspot

ycf3-trnS-GGA

LSC · 45887-46455 · 569 bp

Back to Alloteropsis

Pi 0.0379

MarkerSeek score 75.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 32
pi: 0.0379
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	711-716 bp	1.0	79.6
ycf3-trnS-GGA_p2	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.4	57.8	661-666 bp	1.0	79.6
ycf3-trnS-GGA_p3	GGCTACAATCGAGGAGGTCT	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.6	58.2	709-714 bp	1.0	78.3
ycf3-trnS-GGA_p4	GGCTACAATCGAGGAGGTCT	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.6	57.8	659-664 bp	1.0	78.3
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGGAGGT	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.4	58.5	663-668 bp	1.0	76.7