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psbK-psbI

LSC · 7368-7754 · 387 bp

Back to Alloteropsis

Pi 0.0423

MarkerSeek score 73.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 24
pi: 0.0423
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TTAGCGTTTGTTTGGCAAGC	ATTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.6	531-540 bp	1.0	80.5
psbK-psbI_p2	TTAGCGTTTGTTTGGCAAGC	AATTTTACTCCTCACGCCCA	45.0	45.0	58.2	57.1	532-541 bp	1.0	76.7
psbK-psbI_p3	TTAGCGTTTGTTTGGCAAGC	GGATTACGTCCTGGGTCATT	45.0	50.0	58.2	57.0	512-521 bp	1.0	75.9
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	AATTTTACTCCTCACGCCCAG	45.0	47.6	58.2	58.3	532-541 bp	1.0	73.4
psbK-psbI_p5	TTAGCGTTTGTTTGGCAAGC	AAATTTTACTCCTCACGCCCA	45.0	42.9	58.2	57.9	542 bp	0.333333	42.9