Hotspot

rps16-trnQ-TTG

LSC · 5612-6749 · 1138 bp

Back to Alloteropsis

Pi 0.028
MarkerSeek score 72.40
Primer available yes
Label rps16-trnQ-TTG

Feature Metadata

label_name
rps16-trnQ-TTG
feature_type
igs
region
LSC
length_bp
1138
variable_sites
47
pi
0.028
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-TTG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1221-1444 bp 1.0 83.4
rps16-trnQ-TTG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1211-1434 bp 1.0 82.3
rps16-trnQ-TTG_p3 TATGTCCTTCAAGTCGCACG CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1225-1448 bp 1.0 82.3
rps16-trnQ-TTG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1215-1438 bp 1.0 81.3
rps16-trnQ-TTG_p5 TATGTCCTTCAAGTCGCACG TACTCGGAGGTTCGAATCCT 50.0 50.0 58.0 57.6 1227-1450 bp 1.0 80.5