Hotspot

psbA

LSC · 303-1364 · 1062 bp

Back to Allomorphia

Pi 0.0124

MarkerSeek score 45.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 33
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	CTTAGAGCCCTGTCTACGGA	55.0	55.0	58.1	57.9	1737-1753 bp	1.0	82.0
psbA_p2	GATCCACTTGGCTACATCCG	TAGAGCCCTGTCTACGGAAG	55.0	55.0	58.1	57.9	1735-1751 bp	1.0	82.0
psbA_p3	GATCCACTTGGCTACATCCG	TCTTAGAGCCCTGTCTACGG	55.0	55.0	58.1	57.9	1738-1754 bp	1.0	82.0
psbA_p4	CAATCCACTGCCTTGATCCA	CTTAGAGCCCTGTCTACGGA	50.0	55.0	57.9	57.9	1751-1767 bp	1.0	82.0
psbA_p5	CAATCCACTGCCTTGATCCA	TAGAGCCCTGTCTACGGAAG	50.0	55.0	57.9	57.9	1749-1765 bp	1.0	82.0