Hotspot

ndhC-trnV-UAC

LSC · 51710-52438 · 729 bp

Back to Allomorphia

Pi 0.0173

MarkerSeek score 63.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 29
pi: 0.0173
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATGCCAAAAGTGGAATACCA	GGTTCGAATCCGTATAGCCC	38.1	55.0	56.0	57.9	825-852 bp	1.0	59.3
ndhC-trnV-UAC_p2	TCCGGAAATTAGAAATGCCA	GGTTCGAATCCGTATAGCCC	40.0	55.0	55.0	57.9	838-865 bp	1.0	59.3
ndhC-trnV-UAC_p3	GAAATGCCAAAAGTGGAATACCA	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.9	57.9	827-854 bp	1.0	58.1
ndhC-trnV-UAC_p4	TCCGGAAATTAGAAATGCCAA	GGTTCGAATCCGTATAGCCC	38.1	55.0	55.8	57.9	838-865 bp	1.0	57.9
ndhC-trnV-UAC_p5	AATGCCAAAAGTGGAATACCA	CGGTTCGAATCCGTATAGCC	38.1	55.0	56.0	58.3	826-853 bp	1.0	57.5