Hotspot

trnT-GGU-psbD

LSC · 32465-33682 · 1218 bp

Back to Allomorphia

Pi 0.0125

MarkerSeek score 55.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 35
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AGGAGCCCCTTTAACTCAGT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.0	58.0	1366-1378 bp	1.0	82.6
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1348-1360 bp	1.0	82.5
trnT-GGU-psbD_p3	AGGAGCCCCTTTAACTCAGT	AGAGCCACAAAGGTCCAAAG	50.0	50.0	58.0	58.0	1632-1644 bp	1.0	82.1
trnT-GGU-psbD_p4	AGGAGCCCCTTTAACTCAGT	GAAGAGCCACAAAGGTCCAA	50.0	50.0	58.0	58.0	1634-1646 bp	1.0	82.0
trnT-GGU-psbD_p5	AGGAGCCCCTTTAACTCAGT	GAGCCACAAAGGTCCAAAGA	50.0	50.0	58.0	58.0	1631-1643 bp	1.0	82.0