Hotspot

trnQ-UUG-psbK

LSC · 6777-7107 · 331 bp

Back to Allomorphia

Pi 0.0269
MarkerSeek score 56.20
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
331
variable_sites
19
pi
0.0269
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GCTGACTGAGCGGGTTAAAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.3 57.9 1173-1202 bp 1.0 82.7
trnQ-UUG-psbK_p2 GCTGACTGAGCGGGTTAAAT CCGGGATTACGTCTTGGATC 50.0 55.0 58.3 57.8 1657-1685 bp 1.0 81.5
trnQ-UUG-psbK_p3 GCTGACTGAGCGGGTTAAAT AAACTTACAGCAGCTTGCCA 50.0 45.0 58.3 58.3 1176-1205 bp 1.0 80.7
trnQ-UUG-psbK_p4 GCTGACTGAGCGGGTTAAAT AACTTACAGCAGCTTGCCAA 50.0 45.0 58.3 58.3 1175-1204 bp 1.0 80.7
trnQ-UUG-psbK_p5 GCTGACTGAGCGGGTTAAAT ACTTACAGCAGCTTGCCAAA 50.0 45.0 58.3 58.3 1174-1203 bp 1.0 80.7