Hotspot

ccsA

SSC · 107782-108744 · 963 bp

Back to Alloeochaete

Pi 0.00623

MarkerSeek score 49.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 6
pi: 0.00623
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCAATTTTGTAATGGGACCT	GACGTTCAAGGGGTTCTCAA	38.1	50.0	55.1	57.8	1068-1074 bp	1.0	46.9
ccsA_p2	TCCAATTTTGTAATGGGACCTTC	GACGTTCAAGGGGTTCTCAA	39.1	50.0	57.0	57.8	1068-1074 bp	1.0	46.5
ccsA_p3	CCAATTTTGTAATGGGACCTTCT	GACGTTCAAGGGGTTCTCAA	39.1	50.0	57.0	57.8	1067-1073 bp	1.0	46.4
ccsA_p4	TCCAATTTTGTAATGGGACCTT	GACGTTCAAGGGGTTCTCAA	36.4	50.0	55.8	57.8	1068-1074 bp	1.0	45.2
ccsA_p5	TTCCAATTTTGTAATGGGACCT	GACGTTCAAGGGGTTCTCAA	36.4	50.0	55.8	57.8	1069-1075 bp	1.0	45.2