Hotspot

trnL-UAA-trnF-GAA

LSC · 48755-49118 · 364 bp

Back to Alloeochaete

Pi 0.00877

MarkerSeek score 63.40

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 3
pi: 0.00877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	433-455 bp	1.0	79.9
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	450-472 bp	1.0	79.4
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	448-470 bp	1.0	79.4
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TTTTTCTGTGCCAGGAACCA	50.0	45.0	57.5	57.8	455-477 bp	1.0	78.9
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	TTTTCTGTGCCAGGAACCAG	50.0	50.0	57.5	58.3	454-476 bp	1.0	77.6