Hotspot

ycf3-trnS-GGA

LSC · 45357-45966 · 610 bp

Back to Alloeochaete

Pi 0.00492

MarkerSeek score 61.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 3
pi: 0.00492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	797 bp	1.0	78.9
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	747 bp	1.0	78.6
ycf3-trnS-GGA_p3	TTTTTCCCCTGAGGTTGTCG	TTTCCAATGCTACGCCTTGA	50.0	45.0	58.0	57.8	748 bp	1.0	78.6
ycf3-trnS-GGA_p4	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	50.0	50.0	58.0	58.6	746 bp	1.0	75.7
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	757 bp	1.0	75.7