Hotspot

trnR-UCU-rps14

LSC · 37414-37802 · 389 bp

Back to Alloeochaete

Pi 0.0464

MarkerSeek score 69.10

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 18
pi: 0.0464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	455-463 bp	1.0	65.4
trnR-UCU-rps14_p2	AGGTTTAGAAGACCTCTGTCCT	TTACCGGGTGCAACAAGATC	45.5	50.0	57.9	58.2	461-469 bp	1.0	64.7
trnR-UCU-rps14_p3	AGAAGACCTCTGTCCTATCCA	ATGTTTGTTACCGGGTGCAA	47.6	45.0	56.9	58.3	462-470 bp	1.0	64.4
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TGCAACAAGATCCAGTTGGT	47.6	45.0	56.9	57.6	447-455 bp	1.0	64.1
trnR-UCU-rps14_p5	AGAAGACCTCTGTCCTATCCA	GGGTGCAACAAGATCCAGTT	47.6	50.0	56.9	58.4	450-458 bp	1.0	63.9