Hotspot

trnG-UCC-trnT-GGU

LSC · 13890-15318 · 1429 bp

Back to Alloeochaete

Pi 0.00562

MarkerSeek score 55.00

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1429
variable_sites: 8
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	1549-1572 bp	1.0	41.6
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1519-1542 bp	1.0	41.4
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1515-1538 bp	1.0	40.7
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	1516-1539 bp	1.0	40.7
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	39.1	45.0	57.3	56.7	1551-1574 bp	1.0	40.7