Hotspot

rps16

LSC · 4832-6555 · 1724 bp

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Pi 0.00359
MarkerSeek score 42.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1724
variable_sites
13
pi
0.00359
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 CCTTTCAGGATCAGTCGTGG GGCGTTCTGTCTATCCATGG 55.0 55.0 58.0 58.1 2515-2544 bp 1.0 80.0
rps16_p2 CCTTTCAGGATCAGTCGTGG GGGCGTTCTGTCTATCCATG 55.0 55.0 58.0 58.1 2516-2545 bp 1.0 80.0
rps16_p3 CCTTTCAGGATCAGTCGTGG TAAAATGACCGAGGCCTGTG 55.0 50.0 58.0 57.9 2925-2948 bp 0.857143 74.3
rps16_p4 TAAGTGATAGGCCCCACCAA TAAAATGACCGAGGCCTGTG 50.0 50.0 58.0 57.9 2681-2704 bp 0.857143 74.3
rps16_p5 TTAAGTGATAGGCCCCACCA TAAAATGACCGAGGCCTGTG 50.0 50.0 58.0 57.9 2682-2705 bp 0.857143 74.3