Hotspot

ndhC-trnV-UAC

LSC · 54917-56391 · 1475 bp

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Pi 0.00478
MarkerSeek score 63.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1475
variable_sites
15
pi
0.00478
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCGGAAATTACAAATGCCAGG GGTTCGAATCCGTATAGCCC 47.6 55.0 57.8 57.9 1576-1652 bp 1.0 72.0
ndhC-trnV-UAC_p2 CCGGAAATTACAAATGCCAGG CGGTTCGAATCCGTATAGCC 47.6 55.0 57.8 58.3 1577-1653 bp 1.0 70.2
ndhC-trnV-UAC_p3 CCGGAAATTACAAATGCCAGG AGCCCAATGCAACAAAATGC 47.6 45.0 57.8 58.5 1669-1745 bp 1.0 68.7
ndhC-trnV-UAC_p4 TGTTCCTTCACTAATCGGACC GGTTCGAATCCGTATAGCCC 47.6 55.0 57.4 57.9 1604-1680 bp 1.0 68.4
ndhC-trnV-UAC_p5 TGTTCCTTCACTAATCGGACC CGGTTCGAATCCGTATAGCC 47.6 55.0 57.4 58.3 1605-1681 bp 1.0 66.6