Hotspot

psbZ-trnG-GCC

LSC · 39313-40065 · 753 bp

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Pi 0.00506
MarkerSeek score 58.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
753
variable_sites
10
pi
0.00506
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGCTTCTTCTGATGGTTGGT AATCGAACCCGCATCTTCTC 45.0 50.0 57.0 58.1 900-941 bp 1.0 74.5
psbZ-trnG-GCC_p2 TGCTTCTTCTGATGGTTGGT GAATCGAACCCGCATCTTCT 45.0 50.0 57.0 58.1 901-942 bp 1.0 74.5
psbZ-trnG-GCC_p3 GCTTCTTCTGATGGTTGGTCA AATCGAACCCGCATCTTCTC 47.6 50.0 58.2 58.1 899-940 bp 1.0 72.4
psbZ-trnG-GCC_p4 GCTTCTTCTGATGGTTGGTCA GAATCGAACCCGCATCTTCT 47.6 50.0 58.2 58.1 900-941 bp 1.0 72.4
psbZ-trnG-GCC_p5 TGCTTCTTCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 47.6 50.0 58.2 58.1 900-941 bp 1.0 72.4