Hotspot

trnT-GGU-psbD

LSC · 34974-36124 · 1151 bp

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Pi 0.00341
MarkerSeek score 54.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1151
variable_sites
9
pi
0.00341
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TCCCTCCGTAACCAGTCATC 55.0 55.0 58.1 58.5 1283-1288 bp 1.0 76.3
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.1 58.5 1286-1291 bp 1.0 76.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GAAAGAGCAATAGGCCGGAC 55.0 55.0 58.1 58.7 1324-1329 bp 1.0 74.8
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GAAAGAGCAATAGGCCGGAC 50.0 55.0 58.1 58.7 1327-1332 bp 1.0 74.6
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1260-1265 bp 1.0 74.5