Hotspot

trnQ-UUG-trnG-UCC

LSC · 9600-10723 · 1124 bp

Back to Alisma

Pi 0.00407
MarkerSeek score 64.00
Primer available yes
Label trnQ-UUG-trnG-UCC

Feature Metadata

label_name
trnQ-UUG-trnG-UCC
feature_type
igs
region
LSC
length_bp
1124
variable_sites
11
pi
0.00407
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnG-UCC_p1 TCTTGGAGGTTGTGTAATGCT ACGAATCACACTTTTACCACT 42.9 38.1 57.2 55.5 1228-1247 bp 1.0 42.1
trnQ-UUG-trnG-UCC_p2 TCTTGGAGGTTGTGTAATGCT AGAACGAATCACACTTTTACCAC 42.9 39.1 57.2 57.3 1231-1250 bp 1.0 42.1
trnQ-UUG-trnG-UCC_p3 TCTTGGAGGTTGTGTAATGCT GAACGAATCACACTTTTACCACT 42.9 39.1 57.2 57.3 1230-1249 bp 1.0 42.1
trnQ-UUG-trnG-UCC_p4 TCTTGGAGGTTGTGTAATGCT AACGAATCACACTTTTACCACT 42.9 36.4 57.2 56.2 1229-1248 bp 1.0 42.1
trnQ-UUG-trnG-UCC_p5 TCTTGGAGGTTGTGTAATGCTT ACGAATCACACTTTTACCACT 40.9 38.1 57.9 55.5 1228-1247 bp 1.0 42.1