Hotspot

ycf1

SSC · 108641-113944 · 5304 bp

Back to Alfredia

Pi 0.0017

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5304
variable_sites: 9
pi: 0.0017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACACTAAGCGACGACTCTC	TGGAATTGTCTTTCCTTTTAAAAGT	55.0	28.0	58.1	55.8	285 bp	1.0	40.6
ycf1_p2	ACACTAAGCGACGACTCTCT	TGGAATTGTCTTTCCTTTTAAAAGT	50.0	28.0	58.2	55.8	284 bp	1.0	40.6
ycf1_p3	CACACTAAGCGACGACTCTC	TGGAATTGTCTTTCCTTTTAAAAGTTG	55.0	29.6	58.1	57.6	285 bp	1.0	40.6
ycf1_p4	CACACTAAGCGACGACTCTC	GGAATTGTCTTTCCTTTTAAAAGTTGT	55.0	29.6	58.1	57.6	284 bp	1.0	40.6
ycf1_p5	ACACTAAGCGACGACTCTCT	TGGAATTGTCTTTCCTTTTAAAAGTTG	50.0	29.6	58.2	57.6	284 bp	1.0	40.6