Hotspot

rpl20

LSC · 68483-68863 · 381 bp

Back to Alfredia

Pi 0.00262

MarkerSeek score 45.80

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 381
variable_sites: 1
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GACCTTCCCGGAGTTCATTC	ACTAGGAAAGGGGCAAAAGA	55.0	45.0	58.0	56.3	489 bp	1.0	66.6
rpl20_p2	TATTCGACCTTCCCGGAGTT	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	58.1	56.3	494 bp	1.0	65.7
rpl20_p3	TTATTCGACCTTCCCGGAGT	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	58.1	56.3	495 bp	1.0	65.7
rpl20_p4	GACCTTCCCGGAGTTCATTC	ACTAGGAAAGGGGCAAAAGAA	55.0	42.9	58.0	57.1	489 bp	1.0	64.8
rpl20_p5	CCTTCCCGGAGTTCATTCTC	ACTAGGAAAGGGGCAAAAGA	55.0	45.0	57.7	56.3	487 bp	1.0	64.3