Hotspot

psbC

LSC · 33343-34764 · 1422 bp

Back to Alfredia

Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTTTCTCAGGAAATTCGCGC	GAGGAAACCGAAGTGACTCC	50.0	55.0	58.1	57.9	1579 bp	1.0	78.5
psbC_p2	CGTTTCTCAGGAAATTCGCG	GAGGAAACCGAAGTGACTCC	50.0	55.0	57.9	57.9	1580 bp	1.0	78.2
psbC_p3	GTTTCTCAGGAAATTCGCGC	AGGAAACCGAAGTGACTCCT	50.0	50.0	58.1	58.3	1578 bp	1.0	76.9
psbC_p4	CGGCGGAAGATCCTGAATTT	GAGGAAACCGAAGTGACTCC	50.0	55.0	58.3	57.9	1560 bp	1.0	76.7
psbC_p5	AGGTATTCGTGCTTGGATGG	GAGGAAACCGAAGTGACTCC	50.0	55.0	57.7	57.9	1505 bp	1.0	76.7