Hotspot

psbA

LSC · 473-1534 · 1062 bp

Back to Alfredia

Pi 0.00188

MarkerSeek score 59.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AACAAGAGGGAAGCTATTGC	TTGAAAATTCGTGCGCTTGG	45.0	45.0	55.7	58.2	1180 bp	1.0	60.0
psbA_p2	AACAAGAGGGAAGCTATTGC	ATATTGGTTGACACGGGCAT	45.0	45.0	55.7	57.6	1245 bp	1.0	58.4
psbA_p3	AACAAGAGGGAAGCTATTGC	ATTGGTTGACACGGGCATAT	45.0	45.0	55.7	57.6	1243 bp	1.0	58.4
psbA_p4	AAACAAGAGGGAAGCTATTGC	TTGAAAATTCGTGCGCTTGG	42.9	45.0	56.5	58.2	1181 bp	1.0	58.2
psbA_p5	AAACAAGAGGGAAGCTATTGC	ATTGGTTGACACGGGCATAT	42.9	45.0	56.5	57.6	1244 bp	1.0	56.6