Hotspot

trnL-UAG-rpl32

SSC · 123649-124514 · 866 bp

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Pi 0.00462

MarkerSeek score 61.40

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 866
variable_sites: 4
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	998 bp	1.0	77.3
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	999 bp	1.0	77.3
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	981 bp	1.0	77.1
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	982 bp	1.0	77.1
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	50.0	50.0	57.9	57.6	1008 bp	1.0	76.4