Hotspot

atpB-rbcL

LSC · 54339-55111 · 773 bp

Back to Alfredia

Pi 0

MarkerSeek score 60.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	917-931 bp	1.0	72.8
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	916-930 bp	1.0	72.6
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	844-858 bp	1.0	72.5
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	843-857 bp	1.0	72.2
atpB-rbcL_p5	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	874-888 bp	1.0	72.1