Hotspot

ndhF

SSC · 109649-111874 · 2226 bp

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Pi 0.00835

MarkerSeek score 43.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 75
pi: 0.00835
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGGATCCAACGAAGAAGACC	AGAGTTGGTGAATCGGAAACA	55.0	42.9	58.0	57.5	2533-2536 bp	1.0	70.8
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGAGTTGGTGAATCGGAAACA	50.0	42.9	58.0	57.5	2545-2548 bp	1.0	70.6
ndhF_p3	CCTCAGAAACGGAAGAAGGG	AGAGTTGGTGAATCGGAAACA	55.0	42.9	57.9	57.5	2621-2624 bp	1.0	70.1
ndhF_p4	ACGAAGAAGACCTTTCCCCT	AGAGTTGGTGAATCGGAAACA	50.0	42.9	58.3	57.5	2525-2528 bp	1.0	68.8
ndhF_p5	GGAACAAGAGGGATCCAACG	AGAGTTGGTGAATCGGAAACA	55.0	42.9	58.3	57.5	2542 bp	0.923077	64.9