Hotspot

rpl32-trnL-UAG

SSC · 112949-113711 · 763 bp

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Pi 0.0175

MarkerSeek score 57.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 763
variable_sites: 54
pi: 0.0175
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTTATGGCATGTGTGATGC	TCCCTTTTCCGACGAATCAC	45.0	50.0	56.8	57.9	1387-1461 bp	1.0	77.8
rpl32-trnL-UAG_p2	GTTATGGCATGTGTGATGCAC	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	58.2	57.9	1144-1203 bp	0.923077	72.9
rpl32-trnL-UAG_p3	GTTATGGCATGTGTGATGCAC	TCCCTTTTCCGACGAATCAC	47.6	50.0	58.2	57.9	1403-1460 bp	0.923077	72.3
rpl32-trnL-UAG_p4	TTATGGCATGTGTGATGCAC	TCCCTTTTCCGACGAATCAC	45.0	50.0	56.8	57.9	1402-1459 bp	0.923077	72.1
rpl32-trnL-UAG_p5	GTTATGGCATGTGTGATGCA	TCCCTTTTCCGACGAATCAC	45.0	50.0	56.8	57.9	1403-1460 bp	0.923077	72.0