Hotspot

petD-rpoA

LSC · 76764-76885 · 122 bp

Back to Aletris

Pi 0.018

MarkerSeek score 66.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 9
pi: 0.018
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	ACCACGGCCTAGGTATCTAG	GGACACTTTACATAAGCATTTTGC	55.0	37.5	57.7	57.4	218-228 bp	1.0	49.1
petD-rpoA_p2	ATACCACGGCCTAGGTATCT	GGACACTTTACATAAGCATTTTGC	50.0	37.5	57.0	57.4	220-230 bp	1.0	48.4
petD-rpoA_p3	GTGAAATACCACGGCCTAGG	GGACACTTTACATAAGCATTTTGC	55.0	37.5	58.1	57.4	225-235 bp	0.923077	46.7
petD-rpoA_p4	GTGAAATACCACGGCCTAGG	GCATTTTGCAATTGATTTACCT	55.0	31.8	58.1	54.5	210-220 bp	0.923077	44.6
petD-rpoA_p5	TGAAATACCACGGCCTAGGT	GGACACTTTACATAAGCATTTTGC	50.0	37.5	58.4	57.4	224-234 bp	0.923077	44.2