Hotspot

trnL-UAA-trnF-GAA

LSC · 47105-47462 · 358 bp

Back to Aletris

Pi 0.0233

MarkerSeek score 66.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 31
pi: 0.0233
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GGAGGAAAATCCGTCGACTT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	460-494 bp	1.0	86.0
trnL-UAA-trnF-GAA_p2	AGGAGGAAAATCCGTCGACT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.4	58.1	461-495 bp	1.0	85.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	436-470 bp	1.0	85.4
trnL-UAA-trnF-GAA_p4	GGAGGAAAATCCGTCGACTT	TACCAACTGAGCTATCCCGA	50.0	50.0	57.6	57.6	422-456 bp	1.0	83.3
trnL-UAA-trnF-GAA_p5	GGAGGAAAATCCGTCGACTT	GAACTGGTGACACGAGGATT	50.0	50.0	57.6	57.5	457-491 bp	1.0	82.8