Hotspot

psbC-trnS-UGA

LSC · 34805-34931 · 127 bp

Back to Aletris

Pi 0.0396

MarkerSeek score 78.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 14
pi: 0.0396
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	244-249 bp	1.0	85.0
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	283-288 bp	1.0	84.4
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	263-268 bp	1.0	83.9
psbC-trnS-UGA_p4	CTTTGTGGGACATTTGTGGC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.8	58.0	326-331 bp	1.0	83.4
psbC-trnS-UGA_p5	CTTTGTGGGACATTTGTGGC	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	57.8	57.9	306-311 bp	1.0	82.9