Hotspot

rpoB-trnC-GCA

LSC · 26245-27423 · 1179 bp

Back to Aletris

Pi 0.0153

MarkerSeek score 65.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 75
pi: 0.0153
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTCCGCCAAGCCATGATTAA	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.9	58.1	1301-1329 bp	1.0	84.4
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCCTGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.7	58.1	1226-1254 bp	1.0	83.5
rpoB-trnC-GCA_p3	TTCCGCCAAGCCATGATTAA	CTTACCCCTTGGCTATGTCG	45.0	55.0	57.9	57.8	1280-1308 bp	1.0	83.1
rpoB-trnC-GCA_p4	CTTCCGCCAAGCCATGATTA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.3	58.1	1302-1330 bp	1.0	82.9
rpoB-trnC-GCA_p5	TCCGCCAAGCCATGATTAAT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.6	58.1	1300-1328 bp	1.0	82.5