Hotspot

rpl32-ndhF

SSC · 123505-124386 · 882 bp

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Pi 0.00147
MarkerSeek score 63.70
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
882
variable_sites
14
pi
0.00147
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 TTTAACGCCGCTCCATATCC GTTGCCGTCGGAAAAAGAAG 50.0 50.0 58.1 58.0 1049-1301 bp 1.0 81.9
rpl32-ndhF_p2 TTTAACGCCGCTCCATATCC TGCCGTCGGAAAAAGAAGAA 50.0 45.0 58.1 58.0 1047-1301 bp 1.0 81.8
rpl32-ndhF_p3 TTTAACGCCGCTCCATATCC TTGCCGTCGGAAAAAGAAGA 50.0 45.0 58.1 58.0 1048-1301 bp 1.0 81.8
rpl32-ndhF_p4 TTTAACGCCGCTCCATATCC CTTTTTGTTGCCGTCGGAAA 50.0 45.0 58.1 57.8 1055-1301 bp 1.0 80.6
rpl32-ndhF_p5 TTTAACGCCGCTCCATATCC TTTGTTGCCGTCGGAAAAAG 50.0 45.0 58.1 57.8 1052-1301 bp 1.0 80.6