Hotspot

trnL-UAG-rpl32

SSC · 122554-123339 · 786 bp

Back to Aldama

Pi 0.00259
MarkerSeek score 56.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
786
variable_sites
18
pi
0.00259
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGATATGGAGCGGCGTTAAA 50.0 50.0 58.0 58.1 908-917 bp 1.0 81.6
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGATATGGAGCGGCGTTAAA 50.0 50.0 58.0 58.1 909-918 bp 1.0 81.5
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GGATATGGAGCGGCGTTAAA 50.0 50.0 57.9 58.1 918-927 bp 1.0 80.6
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC GGATATGGAGCGGCGTTAAA 55.0 50.0 57.8 58.1 917-926 bp 1.0 80.0
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC GGATATGGAGCGGCGTTAAA 55.0 50.0 57.8 58.1 916-925 bp 1.0 80.0