Hotspot

ndhD-ccsA

SSC · 121129-121396 · 268 bp

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Pi 0.0113
MarkerSeek score 64.90
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
268
variable_sites
29
pi
0.0113
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 AGGTATTGGTATGTACCCCGA TGGCGACTCTAGGCTTTCTT 47.6 50.0 57.7 58.7 450-2456 bp 1.0 68.4
ndhD-ccsA_p2 AGGTATTGGTATGTACCCCGA CAATTGTGGCGACTCTAGGC 47.6 55.0 57.7 59.0 456-2462 bp 1.0 66.3
ndhD-ccsA_p3 AGGTATTGGTATGTACCCCGA GCAATTGTGGCGACTCTAGG 47.6 55.0 57.7 59.0 457-2463 bp 1.0 66.3
ndhD-ccsA_p4 AGGTATTGGTATGTACCCCGA TGGCGACTCTAGGCTTTCT 47.6 52.6 57.7 58.0 450-2456 bp 1.0 66.1
ndhD-ccsA_p5 TCAGTTGACAAGGTTGAAGGT TGGCGACTCTAGGCTTTCTT 42.9 50.0 57.4 58.7 413-2457 bp 1.0 66.0